Peptide Reconstitution & Concentration Reference
For laboratory and research use only. The information below is provided strictly for educational and scientific reference. These compounds are not intended for human consumption, diagnosis, or treatment.
This page is a laboratory reference for preparing lyophilised research peptides. It covers solvent selection, reconstitution technique, concentration arithmetic, and storage. Every figure below is a unit conversion derived from vial strength and solvent volume.
This page does not recommend, suggest, or imply any quantity for administration. Regenwell PH supplies research compounds only. We do not provide medical advice, clinical consultation, protocols, or supervision, and nothing here should be interpreted as any of those. Determining what quantity is appropriate for a given experimental design is the responsibility of the researcher.
1. The Only Formula You Need
Reconstitution arithmetic reduces to one relationship:
Concentration (mg/mL) = vial strength (mg) ÷ solvent volume (mL)
A 10 mg vial reconstituted with 2 mL of solvent yields 5 mg/mL. That is the entire calculation. Everything in the table below is this formula applied to each vial strength we stock.
The second thing to understand is the syringe. A U-100 insulin syringe is graduated in units, not millilitres, and 100 units equals 1 mL. So:
- 1 unit = 0.01 mL
- 10 units = 0.10 mL
- 50 units = 0.50 mL
- 100 units = 1.00 mL
Units are a measure of volume, never of mass. The same 10 units drawn from two differently reconstituted vials contain completely different amounts of peptide. This is the single most common source of error in peptide handling, and it is why the table below exists.
2. Choosing a Solvent
| Solvent | Composition | Typical use | Working life once opened |
|---|---|---|---|
| Bacteriostatic water | Sterile water with 0.9% benzyl alcohol | General-purpose default for most peptides. The benzyl alcohol inhibits microbial growth, which permits repeated withdrawal from one vial. | Approx. 28 days refrigerated |
| Phosphate-buffered saline | Buffered isotonic salt solution, no preservative | Where a preservative-free or pH-buffered vehicle is required by the assay, or where benzyl alcohol would interfere. | Single working session |
| Acetic water | Dilute acetic acid solution | Poorly soluble sequences that will not fully dissolve in neutral aqueous solvent. The lowered pH improves solubility for certain hydrophobic peptides. | Single working session |
If a peptide does not fully dissolve in bacteriostatic water after gentle swirling, that is the signal to consider an acidic vehicle - not to shake harder. Detailed comparison is in our solvent selection guide.
3. Reconstitution Technique
- Equilibrate. Allow the vial to reach room temperature before opening. Introducing solvent to cold glass promotes condensation.
- Disinfect. Wipe both the peptide and solvent stoppers with an alcohol swab and let them dry.
- Draw the solvent to your chosen volume, referring to the table below.
- Run it down the wall. Angle the needle so solvent flows down the inside of the vial wall onto the powder. Do not inject directly into the lyophilised cake - the force shears peptide bonds and degrades the compound.
- Swirl, never shake. Rotate gently until dissolved. Shaking denatures peptides and generates foam that traps material. Full dissolution may take several minutes.
- Inspect. The solution should be clear and free of visible particulate. Cloudiness or persistent undissolved material indicates incomplete dissolution or a solvent mismatch.
- Label. Record compound, vial strength, solvent volume, resulting concentration, and reconstitution date directly on the vial. Concentration is not recoverable from an unlabelled vial by inspection.
4. Concentration Conversion Tables
Find your vial strength, then the solvent volume you used. The final two columns show what a given volume withdrawn from that vial contains. These are arithmetic conversions, not recommendations.
| Vial | Solvent added | Concentration | 1 unit (0.01 mL) | 10 units (0.10 mL) |
|---|---|---|---|---|
| BPC-157 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| KPV 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| MOTS-C 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| SS-31 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| Tesamorelin 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| SLU-PP-322 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| 5-Amino-1MQ 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| Epitalon 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| Snap-8 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| Semax 5 mg | 1 mL | 5 mg/mL | 50 mcg | 0.5 mg |
| 2 mL | 2.5 mg/mL | 25 mcg | 0.25 mg | |
| 3 mL | 1.667 mg/mL | 16.7 mcg | 0.167 mg | |
| Selank 5 mg | 1 mL | 5 mg/mL | 50 mcg | 0.5 mg |
| 2 mL | 2.5 mg/mL | 25 mcg | 0.25 mg | |
| 3 mL | 1.667 mg/mL | 16.7 mcg | 0.167 mg | |
| Cagrilintide 5 mg | 1 mL | 5 mg/mL | 50 mcg | 0.5 mg |
| 2 mL | 2.5 mg/mL | 25 mcg | 0.25 mg | |
| 3 mL | 1.667 mg/mL | 16.7 mcg | 0.167 mg | |
| AOD-9604 5 mg | 1 mL | 5 mg/mL | 50 mcg | 0.5 mg |
| 2 mL | 2.5 mg/mL | 25 mcg | 0.25 mg | |
| 3 mL | 1.667 mg/mL | 16.7 mcg | 0.167 mg | |
| Retatrutide 10 mg | 1 mL | 10 mg/mL | 100 mcg | 1 mg |
| 2 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| 3 mL | 3.333 mg/mL | 33.3 mcg | 0.333 mg | |
| Retatrutide 20 mg | 1 mL | 20 mg/mL | 200 mcg | 2 mg |
| 2 mL | 10 mg/mL | 100 mcg | 1 mg | |
| 3 mL | 6.667 mg/mL | 66.7 mcg | 0.667 mg | |
| Tirzepatide 15 mg | 1 mL | 15 mg/mL | 150 mcg | 1.5 mg |
| 2 mL | 7.5 mg/mL | 75 mcg | 0.75 mg | |
| 3 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| Tirzepatide 30 mg | 1 mL | 30 mg/mL | 300 mcg | 3 mg |
| 2 mL | 15 mg/mL | 150 mcg | 1.5 mg | |
| 3 mL | 10 mg/mL | 100 mcg | 1 mg | |
| GHK-Cu 50 mg | 2 mL | 25 mg/mL | 250 mcg | 2.5 mg |
| 5 mL | 10 mg/mL | 100 mcg | 1 mg | |
| 10 mL | 5 mg/mL | 50 mcg | 0.5 mg | |
| GHK-Cu 100 mg | 2 mL | 50 mg/mL | 500 mcg | 5 mg |
| 5 mL | 20 mg/mL | 200 mcg | 2 mg | |
| 10 mL | 10 mg/mL | 100 mcg | 1 mg | |
| KLOW Blend 80 mg | 2 mL | 40 mg/mL | 400 mcg | 4 mg |
| 3 mL | 26.667 mg/mL | 266.7 mcg | 2.667 mg | |
| 5 mL | 16 mg/mL | 160 mcg | 1.6 mg | |
| Glutathione 600 mg | 3 mL | 200 mg/mL | 2000 mcg | 20 mg |
| 5 mL | 120 mg/mL | 1200 mcg | 12 mg | |
| 10 mL | 60 mg/mL | 600 mcg | 6 mg | |
| Glutathione 1500 mg | 5 mL | 300 mg/mL | 3000 mcg | 30 mg |
| 10 mL | 150 mg/mL | 1500 mcg | 15 mg | |
| NAD+ 500 mg | 2 mL | 250 mg/mL | 2500 mcg | 25 mg |
| 5 mL | 100 mg/mL | 1000 mcg | 10 mg | |
| 10 mL | 50 mg/mL | 500 mcg | 5 mg |
For any combination not listed, apply the formula in section 1. Vials supplied as ready-to-use solutions - Lipo-C, Lemon Bottle, and L-Carnitine - require no reconstitution and are supplied at their stated volume.
5. Storage and Stability
| State | Temperature | Typical stability |
|---|---|---|
| Lyophilised, sealed | -20°C (long term) | Months to years |
| Lyophilised, sealed | 2-8°C (working stock) | Weeks to months |
| Reconstituted, bacteriostatic water | 2-8°C | Approx. 28 days |
| Reconstituted, preservative-free | 2-8°C | Single working session |
Additional handling notes:
- Do not freeze reconstituted solution. Ice crystal formation and freeze-thaw cycling degrade peptides in solution. Freezing applies to lyophilised powder only.
- Protect from light. Several compounds are photosensitive - GHK-Cu and glutathione notably so.
- Minimise temperature cycling. Return vials to refrigeration promptly rather than leaving them at ambient temperature between withdrawals.
- Humidity matters in the Philippine climate. Lyophilised peptides are hygroscopic and draw moisture from ambient air. Keep vials sealed until the moment of reconstitution.
Our storage and cold chain guide covers tropical-climate handling in more depth.
6. Common Errors
- Treating units as mass. “10 units” is a volume. Without knowing the concentration, it corresponds to no particular quantity of peptide.
- Injecting solvent into the cake. Direct high-velocity contact damages the compound. Always down the wall.
- Shaking to speed dissolution. Causes denaturation and foaming losses. Gentle swirling and patience.
- Failing to label. Two identical-looking vials at different concentrations is an unrecoverable situation.
- Ignoring net peptide content. Vial mass includes residual water and counter-ion. Where precision matters, work from the net peptide figure on the Certificate of Analysis rather than the nominal label weight. Our COA guide explains how to read it.
Questions
For questions about vial specifications, batch documentation, or storage, contact us. We can answer questions about the compounds we supply and the documentation accompanying them. We cannot advise on experimental design or on quantities, and we do not provide medical guidance of any kind.
Products are sold strictly for laboratory research and are not for human or animal consumption. Regenwell PH does not provide medical advice, clinical consultation, or supervision.